Tanım
The HieffTM Magnetic Animal Tissue DNA Kit is designed for DNA extraction from 5–15 mg of animal tissue samples. Utilizing unique magnetic beads and a carefully optimized buffer system, this kit maximizes the isolation and purification of high-purity DNA. The extracted genomic DNA exhibits high purity and stable quality, making it suitable for various downstream applications such as PCR and qPCR. Furthermore, this product is compatible with automated extraction instruments, enabling high-throughput nucleic acid purification.
Features
Broad compatibility: Animal tissues, mouse tails/toes, cultured cells
Fast and Simple Workflow: <1-hour protocol per sample
High-purity DNA: PCR & NGS ready
Components
|
Components No. |
Components Name |
18391ES48 (48 T) |
|
|
Part Ⅰ |
18391-A |
Proteinase K |
1.1 mL/vial × 1 |
|
18391-B |
RNase A |
6 mg/vial × 1 |
|
|
Part Ⅱ |
18391-C |
RNase A Solubilization Buffer |
1 mL/vial × 1 |
|
18391-D |
Lysis Buffer |
11 mL/bottle × 1 |
|
|
18391-E |
Lysis & Binding Buffer* |
25 mL/bottle × 1 |
|
|
18391-F |
Magnetic Bead Suspension |
1.1 mL/vial × 1 |
|
|
18391-G |
Wash Buffer A* |
27.5 mL/bottle × 1 |
|
|
18391-H |
Wash Buffer B* |
20 mL/bottle × 1 |
|
|
18391-I |
Wash Buffer C* |
8 mL/bottle × 1 |
|
|
18391-J |
Elution Buffer |
6 mL/bottle × 1 |
|
[Note]: 18391-E Lysis & Binding Buffer: Add 9.25 mL isopropanol before use.
18391-G Wash Buffer A: Add 11 mL isopropanol before use.
18391-H Wash Buffer B: Add 20 mL absolute ethanol before use.
18391-I Wash Buffer C: Add 32 mL absolute ethanol before use.
Storage
Part I: Proteinase K and RNase A — Store at 2–8 °C; ship at ambient temperature. Shelf life: 18 months.
Part II: Store at ambient temperature; ship at ambient temperature. Shelf life: 18 months.
Figures
1. High-quality & High-purity DNA
Figure 1. Mouse tail DNA extraction results.
a. RNase-treated DNA showed A260/280 and A260/230 ratios around 1.9, indicating good purity.
b. With normal mouse tail size, average DNA concentration was 106.3 ng/µL, suitable for downstream use.
C. Gel electrophoresis band intensity correlated well with measured DNA concentrations.
Documents:
Safety Data Sheet
Manuals
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Sorgu
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