SuperMethyl™ Fast Bisulfite Conversion Kit, Spin Column Purification _ 17329ES

YeasenSKU: 17329ES24

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Satış fiyatı$175.00

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Tanım

The SuperMethyl™-Fast Bisulfite Conversion Kit from Ellis Bio offers the revolutionary fastest and most efficient bisulfite conversion solution available for DNA methylation analysis. Featuring a newly engineered, ready-to-use fast bisulfite conversion reagent and a spin-column DNA purification method, this kit streamlines the workflow by combining conversion and purification in one seamless process. Simply add the SuperMethyl™-Fast Bisulfite Conversion Reagent to your sample and incubate for 6-8 minutes at 98 °C.

Complete bisulfite conversion and DNA purification in under 35 minutes, this kit not only delivers speed but also precision, achieving over 99.5% conversion efficiency while minimizing DNA degradation. Its versatility makes it indispensable for both research and clinical applications.

With an unmatched rapid workflow and exceptional performance, the SuperMethyl™-Fast Bisulfite Conversion Kit ensures reliable and reproducible results, making it the go-to choice for researchers who require both precision and quick turnaround times.

Features

  • Fastest Conversion Available:10X faster than leading industry kits
  • Exceptional Performance:Excellent methylation protection; Compatible with a wide range of DNA inputs.
  • Versatile Applications:Optimized for PCR, microarrays, endonuclease digestion, and next-generation sequencing (NGS).
  • Cost-Effective:At just $179.99, competitively priced with a price-match guarantee.

Components

Component

Volume and Ǫuantity

SuperMethyl - Fast Conversion Reagent

1.5 mL x 7 vials

SuperMethyl - Fast Binding Buffer

28 mL

SuperMethyl - Fast Wash Buffer*

7 mL*

SuperMethyl - Fast Desulphonation Buffer

11 mL

SuperMethyl - Fast Elution Buffer

1.5 mL

SuperMethyl - Spin Columns C Collection Tubes

50 pairs

* SuperMethyl - Fast Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use.

Shipping Conditions

No Cold Chain Required; Shipped at Room Temperature

Precautions / Handling Instructions

Upon receipt of the kit, store the Conversion Reagent at 4°C.

Figures

1.

2.

mESC gDNA converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. C-to-T conversion ratios were determined post-sequencing using unmethylated λ-DNA spike-in control.
3.

mESC gDNA with 1% unmethylated lambda DNA spike-in were converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. False positive rates were calculated post-NGS as the percentage of CpG and non-CpG cytosines showing >1% unconverted C.

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