SuperMethyl™ Max Bisulfite Conversion Kit _ 17328ES

YeasenSKU: 17328ES24

Size: 24 Reactions
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Satış fiyatı$185.00

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Tanım

The SuperMethyl™ Max Bisulfite Conversion Kit from Ellis Bio offers the exceptionally mild yet powerful bisulfite conversion for DNA methylation analysis. Featuring a newly engineered ultra-mild bisulfite conversion formulation and a magnetic bead-based DNA purification method, this kit simplifies the methyl conversion workflow and achieves efficient conversion with minimal DNA degradation. Complete bisulfite conversion and DNA purification can be accomplished in 3 hours or less, preserving DNA integrity even with ultra-low input samples, while consistently achieving 99.8% conversion efficiency. Its broad applicability makes it a valuable tool for both research and translational settings.

Designed for compatibility and consistent performance in sensitive and/or low-input DNA samples, the SuperMethyl™ Max Bisulfite Conversion Kit delivers reliable, reproducible results, making it the preferred choice for precision-focused researchers.

Features

  • Ultra-Mild Conversion Chemistry:Delivers excellent DNA library yield and larger library insert sizes, preserving sample integrity.
  • Low-Input Friendly:Efficiently processes as little as 100 pg of DNA, ideal for limited or precious samples.
  • Exceptional Conversion Efficiency:Achieves 99.8% C-to-T conversion, ensuring unmatched accuracy in methylation analysis.
  • Uniform GC Coverage:Provides more even GC representation, enhancing coverage across promoters and GC-rich regions.
  • Simplified, Rapid Workflow:Up to 3× faster with 5× less hands-on time than enzyme-based methods.
  • Convenient Bead-Based Purification Format:Streamlined purification for improved yield and ease of use. Automation liquid handler friendly.

Component

Component

Volume/Ǫuantity

Storage

Kit Capacity

24 Tests

Please see storage recommendations

for individual components

Conversion Reagent 

2 mL

4 °C (recommended)

Preparation Reagent 

120 µL

Room temperature

Enhancer A 

120 µL

Room temperature

Enhancer B 

240 µL

4 °C (recommended)

Binding Buffer

22 mL

Room temperature

Wash Buffer (concentrate)*

7 mL*

Room temperature

Desulphonation Buffer

6 mL

Room temperature

Elution Buffer

1 mL

Room temperature

Purification Beads 

400 µL

Room temperature

* Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use.

Shipping Conditions

No Cold Chain Required; Shipped at Room Temperature

Precautions / Handling Instructions

Upon receipt of the kit, store the Conversion Reagent and Enhancer B at 4°C.

Figures

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