Tanım
The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a kit capable of performing isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve efficient, rapid, and highly specific detection reactions. It also supports room-temperature reaction system preparation. The buffers and enzyme components in the kit do not contain glycerol and can be lyophilized using the provided lyophilization protectant.
Features
- Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
|
Components No. |
Name |
16730ES60 |
16730ES80 |
|
16730-A |
4×Lyo LAMP Buffer |
600 µL |
6 mL |
|
16730-B |
Bst Plus DNA Polymerase(Glycerol Free) |
100 µL |
1 mL |
|
16730-C |
RT Enzyme mix(Glycerol Free) |
50 µL |
500 µL |
|
16730-D |
25×Fluorescent Dye |
100 µL |
1 mL |
|
16730-E |
Lyophilization stabilizer |
800 µL |
8 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Application
Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow.
2. Consistent performance between lyophilized and liquid formats.
Figure 2. Comparable Detection Performance of Liquid and Lyophilized Formats.
Both liquid and lyophilized versions of the RT-LAMP assay exhibit consistent sensitivity and specificity in detecting DNA and RNA targets, with similar amplification curves and reaction times.
Documents:
Safety Data Sheet
Manuals
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Sorgu
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SSS
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