FuniCut™ HpaII _ 15063ES

YeasenSKU: 15063ES70

Size: 200 T
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Satış fiyatı$35.00
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FuniCut™ restriction enzymes are rapid restriction endonucleases developed through genetic engineering and recombinant technology. They can accurately complete DNA digestion within 5–15 min, and are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, etc. The FuniCut™ series of rapid restriction endonucleases share a single digestion buffer, simplifying the digestion reaction system. In addition, they exhibit excellent enzyme activity redundancy, enabling easy handling of excessive substrates or difficult templates.

Component Information

Component No.

Component Name

15063ES70

15063-A

FuniCut™ HpaII

200 μL

15063-B

10× FuniCut™ Buffer

2×1 mL

15063-C

10× FuniCut™ Color Buffer*

2×1 mL

Product Information

Catalog No.

15063ES70

Size

200 T

Recognition Site

5'-C↓CGG-3'
3'-GGC↑C-5'

Recommended Reaction Conditions

1× FuniCut™ Buffer; optimal incubation at 37°C

Activity

20 U/μL

Inactivation Conditions

Incubate at 80°C for 20 min

Isoschizomers

MspI, HapII, BsiSI

*10× FuniCut™ Color Buffer contains red and yellow tracking dyes, allowing the product to be directly used for gel electrophoresis. The red dye in FuniCut™ Color Buffer migrates at a rate similar to that of a 2500 bp double-stranded DNA fragment in a 1% agarose gel; the yellow dye migrates at a rate similar to that of a 10 bp double-stranded DNA fragment in a 1% agarose gel.

Storage

The product should be stored at -25°C to -15°C. Stable for 2 years.

Notes

1. Prolonged digestion may result in star activity. No star activity was observed after 3 h incubation.

2. Cleavage may be blocked by CpG methylation.

3. For your safety and health, please operate with lab coats and disposable gloves.

4. This product is for research use only.

Documents:

Safety Data Sheet

Storage

The product should be stored at -25°C to -15°C. Stable for 2 years.

FAQ

Citations

Documents

Applications

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