Frequently Bought Together
FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes.This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Specifications
|
Enzyme |
Xbal |
|
Cutting Site |
5'-T↓CTAGA-3' 3'-AGATC↑T-5' |
|
Optimal Reaction Temperature |
37℃ |
|
Sensitive to Heat Inactivation |
YES |
|
Type lls RE |
NO |
|
Methylation Sensitivity |
Not CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive,Not EcoKl Methylation-Sensitive, Not EcoBI Methylation-Sensitive |
|
Star Activity |
3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
|
Isoschizomers |
NO |
Components
|
Components No. |
Name |
15033ES76 |
|
15033-A |
FuniCut™ Xbal |
500 μL |
|
15033-B |
10xFuniCut™ Buffer |
3 x 1 mL |
|
15033-C |
10xFuniCut™ Color Buffer* |
3 x 1 mL |
【Note】: 10× FuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
Storage
FAQ
Citations
Applications
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SSS
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