Tanım
DNase I, or Deoxyribonuclease I, is an endonuclease that can digest single-stranded or double-stranded DNA. It hydrolyzes phosphodiester bonds to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, and Zn2+. In the presence of Mg2+, DNase I can randomly cut any site of double-stranded DNA; in the presence of Mn2+, DNase I can cut the DNA double strand at the same site, forming a blunt end or a sticky end with 1-2 nucleotides protruding, which can be used for the treatment of various RNA samples.
Features
- Recombinant source, yeast
- RNase-free
- High enzymatic cleavage efficiency.
- More suitable for applications sensitive to RNase.
Applications
Removal of contaminating genomic DNA from RNA samples
Degradation of DNA templates in transcription reactions
Removal of gDNA before RNA extraction or reverse transcription.
Removal of template DNA in in vitro transcription.
Specifications
|
Cat.No. |
Size |
Price |
|
14550ES50 |
50 T |
65 |
|
14550ES72 |
250 T |
325 |
Components
|
Components No.
|
Name |
14550ES50 |
14550ES72 |
|
Recombinant DNase I (RNase-free, Yeast) |
150 μL |
750 μL |
|
|
14550-B |
DNase I Reaction Buffer (10×) |
1 mL |
5×1 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for two years.
Figures
RNA extraction application verification
Figure 1 RNA extraction application verification
20 U of the enzyme preparation was used to treat 12 pre-treatment samples of mouse liver from different sources. RNA extraction and agarose gel electrophoresis analysis showed that the RNA integrity of the enzyme-treated group was good, indicating that this DNase I can effectively digest DNA without affecting the quality of RNA, and fully meets the technical requirements of RNA extraction experiments.
Documents:
Safety Data Sheet
Manuals
14550_Manual_Ver.EN20250514.pdf
Citations & References:
Related Blog：
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