Hieff NGS^TM MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) 2.0 utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.

Features

• Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
• Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
• High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
• Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.

Components

Applications

• Gene Expression Research
• Alternative splicing analysis
• Detection and discovery of LncRNA(Non coding RNA)
• dentification of selective polyadenylation sites
• Fusion gene detection

Storage

This product should be stored at -25~-15℃ for 12 months.

Figures

1. Broad Compatibility Across Input Amounts and Sample Types

 Figure 1. Efficient rRNA Removal Across Multiple Sample Types and Input Amounts

Library preparation was performed using 12726 and RNA Library Preparation Kit (Cat#12308, with strand-specific protocols) on various RNA sample types—including human, mouse, and rat total RNA—across different input amounts (100 ng to 1 μg). The rRNA residual rate remained consistently low and was significantly reduced compared to Supplier V* across all sample types and input levels.

2. Effective rRNA Removal

 Figure 2. Comparison of rRNA Removal Efficiency by Sequencing and qPCR

Using 1 μg of 293T total RNA, samples were processed by oligo-dT enrichment or rRNA depletion. Sequencing measured residual rRNA%(A). qPCR targeting cytoplasmic (18S, 28S, 5.8S) and mitochondrial (12S, 16S) rRNAs showed significant Ct value increases after depletion(B), demonstrating effective rRNA removal.

3. Stability

 Figure 3. Stability Test

(A) 1  μg RNA standard was used to compare rRNA removal efficiency between Yeasen (Cat#12726ES) and Supplier V* after storage at 4 °C for 3 days or freshly prepared. Yeasen’s kit maintained normal removal efficiency and outperformed the competitor. rRNA levels were assessed by qPCR targeting 18S, 28S, and ACT.

(B) RNA standard (547 ng/μL) was fragmented (94 °C, 7 min) and RNA Libraries were prepared using Yeasen Kit (Cat#12308, with strand-specific protocols). rRNA removal remained stable after 11 days at 4 °C, comparable to freshly prepared reagents.

Documents:

Safety Data Sheet

12726_MSDS_HB250805_EN.PDF

Manuals

12726_Manual_Ver.EN20250805.pdf