Tanım
The 1× ssDNA Assay Kit is a simple, sensitive, and accurate fluorescent quantification kit for single-stranded DNA (ssDNA), demonstrating excellent linearity within the range of 1–200 ng. This kit includes a fluorescent detection working solution and corresponding ssDNA standards. The assay is straightforward: simply mix the detection working solution with the ssDNA sample and read using a fluorescence microplate reader or a Qubit fluorometer. While the kit does not exhibit higher selectivity for ssDNA over double-stranded DNA (dsDNA), it shows good tolerance to common contaminants such as proteins, salts, and detergents.
Features
Ready to Use: Pre-mixed and stabilized—no preparation needed.
High Sensitivity: Detects as low as 50 pg/µL DNA.
Strong Linearity: Accurate quantification across 0–200 ng.
Stable Signal: Optimized dye and buffer provide consistent fluorescence, even with common contaminants (salts, nucleotides, solvents, detergents, proteins).
Long-Term Stability: Maintains performance for 9 months at 4°C or 25°C.
Broad Compatibility: Works with oligos, PCR primers, denatured dsDNA, and ss circular DNA.
Specifications
|
Assay |
Description |
|
Assay |
ssDNA Quantitation |
|
Excitation / Emission |
500 / 525 nm |
|
For Use With (Equipment) |
Qubit Fluorometer; fluorescence microplate readers |
|
No. of Reactions |
100T / 500T |
|
Product Line |
Qubit Quantitation |
|
Quantitation Range |
1–200 ng; 50 pg/µL – 200 ng/µL |
|
Sample Volume |
1 µL – 20 µL |
|
Detection Method |
Fluorescent |
Components
|
Components No. |
Name |
12646ES60 |
12646ES76 |
|
12646-A |
1×ssDNA Working Solution |
1×concentrate in DMSO |
50 mL |
|
12646-B |
ssDNA Standard 1 |
0 ng/μL in TE buffer |
1 mL |
|
12646-C |
ssDNA Standard 2 |
20 ng/μL in TE buffer |
1 mL |
Storage
This product should be stored at 2~8℃ for 1 year. Protect from light and avoid repeated freeze–thaw cycles.
Application
ssDNA quantification
MGI Circular library and DNB quantification
Figures
1. Good linearity
Figure 1. Linearity assessment across the ranges of 0–200 ng and 0–10 ng.
2. Contaminant tolerance
MgCl2 significantly interferes with the measurement, whereas other impurities have minimal effect. The dye preferentially binds ssDNA but is not strictly ssDNA-specific; therefore, dsDNA contamination in samples should be minimized.
3. Strong Stability
Figure 2. Stability assessment of the 12625 ssDNA Assay Kit and 12646 1× ssDNA Assay Kit after 6 and 9 months of storage at 4°C and 25°C. Both kits maintain consistent performance and stable fluorescence under these conditions.
Documents:
Safety Data Sheet
Manuals
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