Alternative Names

CD44, CDw44, Epican, ECMR-III, LHR, MDU2, MDU3, MIC4, PGP-I, CSPG8, HCELL, HUTCH-I, IN, MC56

Species

Cynomolgus

Expression Sequence

Gln21-Pro220 & Gln387-Ala428

Accession

A0A2K5VJE0

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 27.77 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Cynomolgus CD44, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.