Alternative Names

IL23 alpha, IL12 beta, IL23 alpha&IL12 beta , IL12 β, IL23 alpha&IL12 β

Species

Human,Mouse

Expression Sequence

Arg20-Pro189 (Human IL-23 alpha) and Met23-Ser335 (Mouse IL-12 beta)

Accession

Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Biotinylated Human IL-23 alpha & Mouse IL-12 beta, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 28.1ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.