Alternative Names

UNQ3115, PRO10196, FGF-21, FGF21

Species

Hamster

Expression Sequence

Arg29-Ser209

Accession

XP_005084765.1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 20.80 kDa. Due to glycosylation, the protein migrates to 21-30 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

SPR Data:Hamster FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 264.50 nM as determined in SPR assay .

Reconstitution Method

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.