Product Alias

MHC, HLA-A*02:01, Peptide Ready

Species

Human

Expression sequence

Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M)

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)

Label

C-His-Avi

Expression system

HEK293

Endotoxin

< 1.0 EU per μg by the LAL method.

Preparation

Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized PE-Labeled Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.

Wavelength

Excitation Wavelength: 488 nm/561 nm Emission Wavelength: 575 nm