Alternative Names

SCFR, PBT, CD117, ckit, c-kit, EC 2.7.10, EC 2.7.10.1, KIT

Species

Human

Expression Sequence

Gln26-Pro520

Accession

P10721-2

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 58.60 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Human SCF, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8μg/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.