Alternative Names

CD 24, CD24 molecule, CD24A, FLJ22950, FLJ43543, MGC75043

Species

Human

Expression Sequence

Ser27-Gly59

Accession

P25063-1

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Biotinylated Human CD24, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.