Alternative Names

αVβ5, Integrin alpha V beta 5, beta5, alphaV, ITGAV&ITGB5Integrin alpha V β 5, β5

Species

Human

Expression Sequence

Phe31-Val992(ITGAV) acidic tail&Gly24-Asn719(ITGB5) basic tail

Accession

P06756-1(ITGAV)&P18084(ITGB5)

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 114 kDa (ITGAV)&81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Anti-alpha V Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB5, His Tag with the EC50 of 0.29ug/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.