Alternative Names

FcR-like protein 5, FcRH5, FcRL5, BXMAS1, BXMAS1MGC119592, CD307, CD307e, DKFZp667E2019, DKFZp667F216, FCRH5MGC119593, FLJ00333, FLJ00397, IFGP5, IRTA2

Species

Human

Expression Sequence

Gln16-Gly851

Accession

AAK93971

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Anti-FcRH5 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 46.0ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.