Alternative Names

VEGFC, VEGFc, Flt4-L, VRP, VEGF-C

Species

Human

Expression Sequence

Thr103-Arg227

Accession

Q6FH59

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

ELISA Data: Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA .

Reconstitution Method

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0).