Alternative Names

N2DL-4, NKG2DL4, RAE-1-like transcript 4, RAET1E, LETAL, N2DL4, ULBP4,

Species

Human

Expression Sequence

His31-Asp225

Accession

Q8TD07-1

Tag

C-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 49.12 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【SPR Data】Human ULBP-4, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.17 μM as determined in SPR assay .

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.