Alternative Names

Integrin alpha V beta 1,αVβ1,ITGAV&ITGB1

Species

Human

Expression Sequence

Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail

Accession

P06756-1(ITGAV)&P05556-1(ITGB1)

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human ITGAV&ITGB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.