Product Alias

CT6.1,CTAG1,MY-ESO-1,MHC

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC

Label

C-His-Avi

Expression system

E.coli

Molecular weight

The protein has a predicted MW of 258 kDa. The protein migrates to 160-250 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on SDS-PAGE result under Non reducing (N) condition.

purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22 μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.