Alternative Names

FSH alpha & FSH beta,FSH alphaβFSH alpha,beta

Species

Human

Expression Sequence

Ala25-Ser116 (FSH alpha) & Asn19-Glu129 (FSH beta)

Accession

P01215(FSH alpha)&P01225 (FSH beta)

Tag

C-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 10.20 kDa (FSH alpha) and 39.2 kDa (FSH beta). Due to glycosylation, the protein migrates to 25-30 kDa (FSH alpha) and 50-60 kDa (FSH beta) based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution Method

1. Centrifuge before opening2. Reconstitute with distilled water (>100 μg/mL)3. Aliquot to avoid freeze-thaw cycles