Property Name

Property Details

Alternative Names

E-Cadherin, Epithelial cadherin, Cadherin1, Cadherin-1

Species

Human

Expression Region

Asp155–Ala709

Accession

P12830-1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 61.62 kDa. Due to glycosylation, the protein migrates to 70–80 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE, > 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution

Centrifuge the vial before opening. Reconstitute lyophilized protein using distilled water at a concentration above 100 μg/mL. It is recommended to aliquot the reconstituted protein for single use to avoid repeated freeze-thaw cycles.