Synonyms

CHE1;BCHE;Cholinesterase

Source

Human BCHE/Butyrylcholinesterase Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu29-Leu602.[Accession | P06276]

Molecular Weight

The protein has a predicted MW of 66.18 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE

Formulation

Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM Nacl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.