Synonyms

APOE;APOE3;Apo-E;Apolipoprotein E

Source

Human APOE3/Apolipoprotein E Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys19-His317.[Accession | P02649]

Molecular Weight

The protein has a predicted MW of 59.76 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE

Activity

SPR Data:Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay .

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.