Source

Recombinant Mouse IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr29-Ala193.[Accession | Q9Z0M9]

Molecular Weight

The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Activity

SPR Data: Mouse IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.86 nM as determined in SPR assay (Biacore T200)

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.