Synonyms

NF-KB; Receptor activator of NF-KB;ODFR; CD265

Source

Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Val31-Ser214.[Accession | O35305]

Molecular Weight

The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.