Synonyms

PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD

Uniprot No.

P00749-1

Source

Human PLAU/uPA Protein (active form) is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ser21-Leu431, which consists of two chains: Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is further cleaved to yield a short chain A (Lys156-Phe 177) and N-terminal fragment (Ser21-Lys155).

Molecular Weight

The protein has a predicted MW of 47.5 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Tris-Bis PAGE result.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% as determined by SDS-PAGE and HPLC

Bioactivity

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-

4-methylcoumarin (Z-GGR-AMC). The specific activity is > 2000 pmol/min/μg

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended. Dissolve the lyophilized protein in distilled water.