Synonyms

notch ligand DLL4; MGC126344;DLL4; Delta-like 4

Source

Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser27-Pro524.[Accession | Q9NR61]

Molecular Weight

The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

ELISA Data: Immobilized Human DLL4, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA .

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.