Product Alias

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-feto, AFP, HPAFP, AFPD, FETA, MHC

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*02:03),Ile21-Met119(B2M) and FMNKFIYEI peptide

Accession

AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 50.70 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.