Product Alias

MHC, HLA-A, P53, TP53, Antigen NY-CO-13, BCC7, FLJ92943, LFS1, TRP53

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC

Label

C-His-Avi

Expression system

HEK293

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22 μm filtered solution in 20 mM PB, 500 mM NaCl, 0.2% BSA (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized PE-Labeled Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.

wavelength

Excitation Wavelength: 488 nm/561 nm Emission Wavelength: 575 nm