Product Alias

HLA0201, MHC I, gp100, Glycoprotein 100, ME20-M, P100

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide

Accession

A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GP100 (HLA-A*02:01) , His Tag with the EC50 of 0.88μg/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.