คำอธิบาย
The Human Naïve T Cell Expansion Cytokine Set contains recombinant human IL-2, recombinant human IL-7, and recombinant human IL-15. These three cytokines are essential key growth factors for the culture of Naïve T cells. This combination minimizes the generation of terminally differentiated effector T cells and exhausted T cells, and the expanded cells possess stronger self-renewal capabilities and in vivo persistence.
IL-2 is a potent T cell growth factor that drives clonal expansion. On the basis of IL-7/IL-15, it provides additional proliferation signals.
IL-7 is a survival factor, and its main function is to maintain T cell basal metabolism and survival. It is crucial for Naïve T cells and T memory stem cells. It can drive homeostatic proliferation and help maintain the Naïve/T memory stem cell phenotypes such as CD45RA, CCR7, and CD62L.
IL-15 mainly functions to promote slow, homeostatic proliferation and promotes basal proliferation without driving terminal differentiation. It enhances CD8⁺ T cell persistence.
IL-7+IL-15: Mimics homeostatic "tonic" signals, maintains cell viability, promotes slow and steady division, and maintains the naïve phenotype. Low-dose IL-2: Provides stronger proliferation signals upon activation (via TCR/CD3/CD28), and the low dose can reduce cell differentiation.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Human Naïve T Expansion Cytokine Set |
92647ES10 |
10 μg |
|
92647ES50 |
50 μg |
|
|
92647ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92647ES10 |
92647ES50 |
92647ES60 |
Quantity |
|
92647ES-A |
Human IL-2 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92647ES-B |
Human IL-7 Protein |
10 μg |
50 μg |
100 μg |
1 |
|
92647ES-C |
Human IL-15 Protein |
10 μg |
50 μg |
100 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
90213ES |
Human IL-2 Protein |
E.coli |
Lyophilized powder |
|
90188ES |
Human IL-7 Protein |
E.coli |
Lyophilized powder |
|
90113ES |
Human IL-15 Protein |
E.coli |
Lyophilized powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Human IL-2 Protein |
Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL; further dilution can be performed according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human IL-7 Protein |
|
|
Human IL-15 Protein |
Storage
|
Component Name |
Storage Conditions |
|
Human IL-2 Protein |
Store at -25 ~ -15°C, valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C under sterile conditions, valid for 7 days. After reconstitution, store at -85 ~ -65°C under sterile conditions, valid for 3 months. |
|
Human IL-7 Protein |
|
|
Human IL-15 Protein |
Specifications
|
Parameter |
Specification |
|
Source |
Human |
|
Tag |
No Tag |
|
Expression System |
E. coli |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Appearance |
Lyophilized Powder |
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
Documents:
Manuals:
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