Cat.No.

91232ES08 / 91232ES20 / 91232ES60 / 91232ES76

Size

5 μg / 20 μg / 100 μg / 100 μg

Name

91232ES08

91232ES20

91232ES60

91232ES76

HiActi™ Recombinant Mouse IFN-alpha 1a, His Tag

5 μg

20 μg

100 μg

500 μg

Synonyms

interferon alpha 1a, Ifa, Ifa8, IFNA

Species

Mouse

Expression Sequence

CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus.

Accession

P01572

Tag

N-His

Expression System

E.coli

Molecular Weight

The protein has a calculated MW of 19.93 kDa. The protein migrates as 17-25 kDa under reducing condition

Purity

>98% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Formulation

The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.

Appearance

Lyophilized Powder

Biological activity

Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 x 10⁷ IU/mg.

Reconstitution Instructions

Briefly centrifuge the vial at 3000 rpm for 5 minutes before opening.

It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL, and let it stand for at least 20 minutes to ensure complete dissolution.

The reconstituted solution can be further diluted and aliquoted. When performing further dilution and aliquoting, a carrier protein (0.1% BSA, 10% FBS, or 5% HSA) must be added. For serum-free experimental requirements, a 5% trehalose solution can be used as an alternative carrier.