This product utilizes a next-generation dual-antibody blocked hot-start Taq enzyme that simultaneously blocks both the 5'→3' polymerase and 5'→3' exonuclease activities, enabling efficient blocking and precise release of the enzyme. Combined with an exclusively optimized buffer system, ultra-clean manufacturing environment, and stringent quality control standards, the amplification performance and stability of the reagent are further enhanced.

Product Information

Component Information

Feature

• Supports direct amplification from oral swabs: When used with nucleic acid release reagent, direct amplification performance is not affected.
• Excellent stability of fully premixed formulation: Premixed primers and probes remain stable after 7 days at 37°C and 20 freeze-thaw cycles.
• Low background: No detection of 11 pathogens and human genomic DNA across 48 replicates.
• High sensitivity: Stable detection at Ct 37–39, slightly superior to comparable market products.
• Supports fast protocols: Results available within 30 minutes.
• dUTP/UDG contamination prevention system: Incorporates a dUTP/UDG system that effectively prevents aerosol contamination.
• Multi-platform and multi-system compatibility: Compatible with Bio-Rad CFX96, ABI Q5, 7500, SLAN, Tianlong, and other instruments.

Figure

Case Study 1: Direct Amplification from Swab Samples

Throat swab samples were collected and treated with nucleic acid release reagent (Cat#16964) by vortexing. The resulting solution and TE buffer were used separately as template diluents to dilute three mixed plasmid templates to the same concentration. Amplification was performed using both 16945 and comparable market products. Results showed that 16945 exhibited superior tolerance to inhibitors and supports direct amplification from swab samples.

Figure 1. Comparison of direct amplification performance from swab samples between 16945 and comparable market products.

Case Study 2: Pathogen Nucleic Acid Detection

Using the 16945 reagent with 11 pathogen assays and GAPDH primers/probes, water was used as the template, with 48 replicate wells per target. Results showed no non-specific amplification curves, indicating the high cleanliness of the 16945 product.

Case Study 3: Amplification Efficiency Test

Using the 16945 reagent, linear gradient testing was performed on 3 pathogens at concentrations ranging from 10 to 10⁷ copies/μL. Results showed amplification efficiencies all above 96% with R²≥0.99, indicating that 16945 supports multiplex amplification with excellent efficiency.

Figure 2. Linearity test of the 16945 product.

Case Study 4: Customer Cases

Testing was performed on client-provided primers, probes, and samples. Compared to competitor reagents, 16945 showed better stability of the fully premixed formulation at 37°C for 7 days. For 8 African swine fever (ASF) real samples, the detection Ct values were approximately 1 cycle earlier, indicating that 16945 exhibits superior overall performance compared to similar products.

Figure 3. Selected customer case demonstration.

Storage

Store at -25°C to -15°C, valid for 18 months.

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