The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation.

Features

• Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
• High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
• Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
• Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.

Components

Shipping and Storage

This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.

Application

Isothermal Amplification;Rapid detection of pathogenic bacteria.

Figures

1. Workflow

Figure 1. RT-LAMP Workflow.

2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection

Figure 2. Broad Pathogen Detection Compatibility.

(A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets.

(B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity.

3. High Specificity

Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A*

Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification.

4. Strong stability

Figure 4. Stability Evaluation of the RT-LAMP System.

(A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles.

(B) Thermal accelerated stability: Maintains amplification efficiency after 7 days of storage at 37°C.

Documents:

Safety Data Sheet

16729_MSDS_HB250713_EN.PDF

Manuals

16729_Manual_Ver.EN20250708.pdf