Hieff NGS™ Barcode Ligation Module is a ligation module designed for the Native Barcode ligation step in DNA library construction on third-generation nanopore sequencing platforms. Through directed engineering and optimization, the enzyme components in this module rapidly ligate Native Barcodes to end-repaired/dA-tailed products (5′-phosphorylated with a 3′-dA overhang), reducing overall hands-on time. All reagents in this kit undergo rigorous quality control and functional validation, maximally ensuring library consistency and reproducibility.

Features

High‑efficiency native barcode ligation

Delivers robust ligation performance for end‑repaired and dA‑tailed DNA fragments.

Optimized for long‑read library prep

Tailored for nanopore third‑generation sequencing workflows.

Reliable batch‑to‑batch consistency

Strict QC and functional validation guarantee reproducible library outcomes.