The Hieff NGS™ MagSP rRNA Removal Kit (Prokaryote) with Purification Beads uses streptavidin-labeled magnetic beads to deplete ribosomal RNA from prokaryotic (universal across bacteria and archaea) total RNA, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. The kit delivers efficient rRNA removal from both intact and partially degraded total RNA. The rRNA-depleted RNA is suitable for downstream applications including high-throughput sequencing of mRNA and non-coding RNA—which markedly increases the proportion of informative reads—as well as cDNA synthesis and other downstream uses.

Features

• Magnetic-bead rRNA removal workflow.

• Probe set covers both bacteria and archaea.

Applications

1. Probe coverage across prokaryotic species

Figure 1. Probe coverage of the prokaryotic rRNA removal kit across bacterial phyla and archaeal lineages.

2. rRNA removal performance

Figure 2. Residual rRNA (%) comparison between the Yeasen kit (Cat#12265ES) and a competitor kit. 16S and 23S rRNA were quantified by qPCR. The Yeasen kit achieves >99% rRNA removal efficiency.

Storage

Box I (Probe Mix): store at -25 to -15°C. Box II: store at 2 to 8°C.