Hifair^TM Superpro Ⅱ One Step RT-qPCR Master Mix is a one-tube premixed kit for single or multiple quantitative PCR reactions using RNA as a template. During the experiment, reverse transcription and quantitative PCR were carried out in the same reaction tube, which simplified the experimental operation and reduced the risk of pollution. The kit supports primer and probe pre-mixing, allowing for a streamlined workflow—simply add the template to the prepared reaction mix and proceed directly to on-machine amplification.

Features

• Exceptional Premix Stability: Support prime&probe premixing, with proven stability at 37°C for up to 5 days.
• Ultra-High Sensitivity and Specificity: Features a genetically engineered antibody-based hot-start Taq polymerase for enhanced sensitivity and specificity.
• dUTP/UDG Anti-Contamination System: Incorporates a dUTP/UDG system to effectively prevent aerosol contamination.
• Broad Instrument Compatibility: Compatible with a wide range of real-time PCR platforms, including Bio-Rad CFX96, ABI Q5, 7500, and SLAN.

Components

【Note】: The Mix include dNTPs, dUTP, Mg2+, stabilizers, enhancer, Reverse Transcriptase, HotStart Taq DNA Polymerase and UDGase, etc.

Shipping and Storage

This product should be stored at -25~-15℃ for 12 months.

Application

RT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)

Figures

1. High-performance "one-tube" fully premixed RT-qPCR reagents

 Figure 1. Simplify product format and workflow with the HifairTM Superpro Ⅱ One Step RT-qPCR Master Mix

2. Robust amplification and detection of different viral RNA (4 targets)

Figure 2. Multiplex detection (5 targets) with the Hifair^TM Superpro Ⅱ One Step RT-qPCR Master Mix

Multiplex detection of SARS-CoV-2, Influenza A, Influenza B, and internal control (5000 copies/mL and 500 copies/mL) using Yeasen's fully premixed RT-qPCR reagent (11899ES) and supplier T*.

Yeasen's reagent successfully supported at least four-target multiplex amplification without compromising performance at low template concentrations. Results were comparable to the T brand, with slightly earlier Ct values in some channels, indicating a sensitivity advantage.

3. Sensitivity

 Figure 3. Sensitivity test

RT-qPCR targeting Influenza A and Influenza B was performed using the Hifair^TM Superpro Ⅱ One Step RT-qPCR Master Mix. The reagent successfully detected all low-concentration Influenza A and B templates (8/8), demonstrating excellent sensitivity.

4. High GC-content tolerance

 Figure 4. GC tolerance test

Comparison of Yeasen's fully premixed RT-qPCR reagent(11899ES) and traditional reagent (13650) in amplifying 1 µg of 293 RNA templates with 30–60% GC content.

Results show no significant difference between the two reagents across templates with varying GC content, indicating broad GC tolerance of Yeasen’s fully premixed formulation.

5. Ultra-high stability

 Figure 5. Stability comparison of Yeasen's fully premixed RT-qPCR reagent (Cat#11899ES) and competitor T brand with primers and probes added, stored at 37 °C for 5 days.

Yeasen's premixed reagent showed no significant Ct shift when amplifying Influenza A and B templates at 2000 and 200 copies/mL, indicating stable performance. After 5 days at 37 °C, Yeasen (Cat#11899ES) also demonstrated lower Ct values and higher fluorescence signals compared to the T brand, confirming its superior stability.

Documents:

Safety Data Sheet

11899_MSDS_HB250617_EN.PDF

Manuals

11899_Manual_Ver.EN20250407.pdf