The Hieff™ Bacterial DNA Kit is designed for the extraction of genomic DNA from Gram-positive and Gram-negative bacteria. The silica-based matrix used in the spin column is a proprietary novel material. Combined with our unique buffer formulation, it maximizes the removal of impurities such as cellular metabolites and proteins. The extracted DNA is of high purity and stable quality, making it suitable for various downstream applications, including restriction enzyme digestion, PCR, and library construction.
Application
Broad Applicability: Suitable for both Gram-positive and Gram-negative bacteria.
High DNA Purity: Free from protein and RNA contamination, ready for direct use in downstream applications such as PCR, restriction enzyme digestion, and qPCR.
Safe and Eco-friendly: Eliminates the need for toxic organic solvents like phenol and chloroform during extraction.
Components
|
Category |
Components NO. |
Name |
18806ES08 |
18806ES50 |
18806ES70 |
|
Part I |
18806-A |
Proteinase K (20 mg/mL) |
100 μL |
1 mL |
1 mL×4 |
|
Part II |
18806-B |
DNA Binding Column (Hieff™ DNA Column B1) |
5 |
50 |
200 |
|
18806-C |
2 mL Collection Tube B1 |
5 |
50 |
200 |
|
|
18806-D |
Buffer AC (AC Buffer B1 |
500 μL |
5 mL |
20 mL |
|
|
18806-E |
Lysis Buffer LB(LB Buffer B1) |
3 mL |
30 mL |
120 mL |
|
|
18806-F |
Binding Buffer BD (BD Buffer B1) |
1 mL |
10 mL |
40 mL |
|
|
18806-G |
Protein Removal Buffer PL* (PL Buffer B1*) |
1.6 mL |
16 mL |
64 mL |
|
|
18806-H |
Wash Buffer W* (Wash Buffer*) |
1.3 mL |
13 mL |
50 mL |
|
|
18806-I |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Figure
1. Higher Yield
Figure 1. Comparison of gDNA extraction performance between Kit 18806.
Four aliquots of 2 mL E. coli suspension were processed using Kit 18806(Y1/Y2) and a comparable product from Brand T(T1/T2), with gDNA eluted in 50 μL ddH₂O. Subsequently, 5 μL of each eluate was analyzed by gel electrophoresis. The results demonstrate that Kit 18806 yields a higher amount of gDNA with superior integrity compared to Brand T.
Storage
FAQ
(2) Incomplete cell wall disruption or lysis. For plant samples, ensure thorough homogenization and grinding. Avoid improper storage of the lytic enzymes provided in the kit. Pre-warming the elution buffer or performing multiple passes through the column can also help.
(2) Harsh handling during the extraction process may cause mechanical shearing of the DNA.
Solutions: Use fresh starting materials whenever possible and store samples at low temperatures to avoid repeated freeze-thaw cycles. After grinding in liquid nitrogen or tissue homogenization, handle the samples gently during subsequent steps. Avoid repeated freeze-thaw cycles of the DNA elution buffer.
(2) Residual ethanol. Ensure that absolute ethanol is added to the Wash Buffer as instructed, and prevent the ethanol from evaporating prematurely. Perform an additional dry spin (centrifugation of the empty column) to ensure that all residual ethanol is completely removed.
Citations
Applications
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Förfrågan
Vanliga frågor
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