Hieff NGS™ Cap Beads are streptavidin magnetic beads prepared by covalently coupling high-purity streptavidin to hydrophilic superparamagnetic beads. Based on the extremely high affinity of the streptavidin-biotin system, this product can rapidly and efficiently bind biotin-modified nucleic acids, making it suitable for nucleic acid hybridization capture. The beads have uniform particle size, regular morphology, and excellent reproducibility and batch-to-batch stability.

Product Information

Component Information

Storage

Store at 2-8°C. Valid for 1 year.

Instructions for use

Use in conjunction with Hieff NGS™ Hyb & Wash Kit with Universal Blocking Oligo for Illumina (Cat#12245) and Hieff NGS™ Hyb & Wash Kit with Universal Blocking Oligo for MGI (Cat#12243) hybridization capture series products.

Notes

1. Do not store the beads below 0°C. Before use, allow the beads to equilibrate to room temperature.

2. Ensure the bead suspension is thoroughly vortex-mixed before pipetting. Avoid vigorous shaking.

3. Use low-adhesion pipette tips when aspirating beads to prevent loss due to bead adhesion to the tips.

4. Avoid high-speed centrifugation of the beads or prolonged placement on a magnetic rack, which may cause bead aggregation.

5. Excessive drying time may cause bead cracking, affecting capture efficiency.

6. Prolonged incubation may cause partial bead aggregation. Gently flick the tube bottom to resuspend the beads.

7. After adding wash buffer, it is recommended to gently pipette up and down to mix. Avoid generating bubbles.

8. This product is for research use only.

9. For your safety and health, please operate with lab coats and disposable gloves.

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