Описание
The MolPureTM Plant RNA Kit is suitable for extracting RNA from polysaccharide polyphenol plants such as Arabidopsis thaliana, rice, maize, wheat, tomato, tobacco, cotton and Holly. The extraction and purification of total RNA from plant samples (100-200 mg fresh or cryopreserved samples) can be completed within 40 minutes without the use of toxic organic substances such as phenol and chloroform. The silicon matrix material used in the centrifugal adsorption column is a unique new material of our company, which can maximize the recovery of high-purity RNA with our unique lysis solution formula. DNase I directly digests gDNA on the column, and the extracted total RNA has high purity, stable and reliable quality, and can be applied to various downstream application experiments, such as RT-PCR, RT-qPCR, in vitro transcription, molecular cloning etc.
Features
Safety: no need to use organic reagents such as phenol chloroform.
Fast: RNA extraction from samples can be completed within 40 min minutes.
Widely compatibility: It is suitable for RNA extraction from Arabidopsis thaliana, rice, corn, wheat, tomato, tobacco, cotton, holly and other simple polysaccharide and polyphenol plant samples.
Low genome residue: equipped with DNase I, which can effectively remove DNA pollution.
Applications
Plant RNA extraction;Total RNA extraction from plants;
Specifications
|
Cat.No. |
19291ES08 /19291ES50 |
|
Size |
5 T / 50 T |
Components
|
Category |
Components No. |
Name |
19291ES08 |
19291ES50 |
|
Part I |
19291-A |
DNase Buffer |
250 μL |
1.25 mL ×2 |
|
19291-B |
DNase I (RNase-free) |
25 μL |
250 μL |
|
|
Part II |
19291-C |
RNA Adsorption Column P4 (MolPureTM RNA Column P4) |
5 |
50 |
|
19291-D |
2 mL Collection Tube P4 |
5 |
50 |
|
|
19291-E |
Lysate LB (LB Buffer P4) |
5 mL |
50 mL |
|
|
19291-F |
Deproteinized solution PL (PL Buffer P4) |
4 mL |
40 mL |
|
|
19291-G |
Wash Buffer W * |
1.3 mL |
13 mL |
|
|
19291-H |
RNase-free H2O |
1 mL |
5 mL |
Shipping and Storage
Part I should be stored at -25~-15℃ for one year.
Part II should be stored at room temperature for 1 years.
Figures
RNA was extracted from Rice, corn, Arabidopsis, tomato. The extracted RNA was then subjected to agarose gel electrophoresis, and the electrophoretogram is shown below.
Table 1. High quality of extracted products.
Documents:
Safety Data Sheet
Manuals
19291_Manual_Ver.EN20250409.pdf
Citations & References:
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Расследование
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