In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
Yeasen
Cleascrip ™ T7 РНК-полимераза (GMP-класс, низкая дцРНК, 250 ед/мкл) _ 10629es
Цена продажиОт $198.00
Сохранять $40.00
Yeasen
Фермент мРНК вакциния, покрывая фермент GMP-класс (10 U/мкл) _ 10614ES
Цена продажиОт $365.00
