Hieff™ Blood RNA Kit _ 19241ES

YeasenSku: 19241ES50

Size: 50 T
Цена:
Цена продажи$225.00
Запас:
В наличии

The Hieff™ Blood RNA Kit is suitable for the extraction of RNA from samples such as blood, plasma, serum, and lymph fluid. The silica-based material used in the centrifugal adsorption column is a unique new material developed by our company. It, combined with our unique lysis buffer formulation, allows for the maximum recovery of high-purity RNA. The extracted RNA has high purity, stable and reliable quality, and can be used for various downstream applications, such as RT-PCR, RT-qPCR, in vitro transcription, molecular cloning, etc.

Features

Fast and simple: simple operation, single sample extraction is completed in 30 minutes.

Safe and non-toxic: no isopropanol extraction is required.

Application Blood samples: suitable for RNA extraction from blood, plasma, serum, lymph and other samples.

Components

Prat

Components No.

Name

19241ES50

Part I

19241-A

Lysis Buffer / LB Buffer B3

50 mL

Part II

19241-B

Hieff™ RNA Column B3

50

19241-C

2 mL Collection Tube B3

50

19241-D

Deproteinized Solution* / PL Buffer B3*

16 mL

19241-E

Wash Buffer*

12 mL

19241-F

RNase-free H2O

5 mL

Application

RNA extraction from blood, plasma, serum, or lymph samples.

Figures

Whole Blood RNA Extraction

Table 1. Concentration and purity QC results of the extracted RNA

Sample No.

Concentration (ng/μL)

OD260/280

OD260/230

Extraction reagents

1

18.24

1.99

0.92

Yeasen-19241

2

18.14

2.01

1.17

3

25.23

1.76

0.60

4

11.57

1.39

0.14

5

13.54

1.47

0.11

Supplier M*

6

14.51

1.49

0.11

Six 250  μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 1  μL of each eluate was used to measure RNA concentration.

Result: 19241 showed higher RNA yield.

Figure 1. Blood RNA Integrity QC Results

Figure 1. Blood RNA Integrity QC Results

Six 250 μL fresh blood samples were extracted using 19241 and M brand kits, with RNA eluted in 50 μL RNase-free water. 5 μL of each eluate was analyzed for RNA integrity and band intensity.
Result: RNA extracted with 19241 showed better integrity and clearer bands.

Storage

This product should be transported at room temperature. Upon receipt:

Part I: Store at 4°C, protected from light.

Part II: Store at room temperature.

The product is vali d for one year.

FAQ

Citations

Applications

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Расследование

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