Описание
This product is designed for the isolation of total RNA from animal tissues (e.g., liver, kidney, spleen, etc.). It utilizes uniquely engineered magnetic beads and an optimized buffer system to efficiently capture released nucleic acids. The extracted RNA is of high purity, stable, and reliable in quality, making it suitable for downstream applications such as RT-PCR, Northern blotting, and in vitro translation. When used with automated nucleic acid extraction instruments, this kit enables high-throughput RNA purification.
Features
- Wide Sample Coverage: Compatible with various animal tissues and aquatic samples.
- Rapid, Automated Workflow: Complete extraction in <1 hour; fully compatible with Yeasen 16/48/96-channel extractors.
- Pure, DNA-Free RNA: Built-in DNase I ensures clean RNA for RT-PCR and NGS applications.
Components
|
Components No. |
Name |
18605ES50 (50T) |
18605ES60 (100 T) |
|
18605-A |
Lysis Buffer RLT |
30 mL |
60 mL |
|
18605-B |
Protein Removal Buffer PE* |
32 mL |
64 mL |
|
18605-C |
Wash Buffer RW** |
25 mL |
50 mL |
|
18605-D |
Magnetic Beads suspension |
500 μL |
1 mL |
|
18605-E |
RNase-free H₂O |
10 mL |
20 mL |
[Note]:
For 18605ES50, Add 20 mL of absolute ethanol to Protein Removal Buffer PE* (18605-B), and 100 mL of absolute ethanol to the Wash Buffer RW(18605-C).
For 18605ES60, Add 40 mL of absolute ethanol to Protein Removal Buffer PE* (18605-B), and 200 mL of absolute ethanol to the Wash Buffer RW(18605-C).
Storage
This product should be stored at room temperature for 2 years.
Application
Tissue RNA Extraction; High-purity RNA Extraction; Gene Expression Analysis.
Figures
High Yield and High Integrity RNA Extraction
|
Reagent / Kit |
Sample Type |
RNA Concentration (ng/μL) |
|
Yeasen |
Mouse Liver |
612.10 |
|
Mouse Spleen |
1117.27 |
|
|
HEK293 Cells |
1057.58 |
|
|
Supplier O* |
Mouse Liver |
349.88 |
|
Mouse Spleen |
1038.39 |
|
|
HEK293 Cells |
924.74 |
Figure 1. Comparison of RNA Extraction from Diverse Samples
RNA was extracted from 10 mg mouse liver, 10 mg mouse spleen, and 4×106⁶ 293T cells using Yeasen Kit (Cat. No. 18605) on an automated platform or a manual kit from Supplier O. Nanodrop quantification showed higher RNA yields with Yeasen. Agarose gel electrophoresis (3 µL RNA) revealed sharp 28S/18S rRNA bands and no genomic DNA contamination.
Cas Study-Verification of Magnetic Bead Aggregation During RNA Extraction
Background
During high-input RNA extraction workflows, magnetic bead aggregation was occasionally observed. It was hypothesized that excess nucleic acid content contributed to bead clumping. To address this issue, DNase I digestion and surfactant supplementation were evaluated.
Sample Input: Vero cells, ~1 × 10⁶ cells per sample
Kit used: Yeasen HieffTM Magnetic Tissue/Cell Total RNA Kit (18605ES)
Experimental Design
|
Group |
Condition |
Sample ID |
|
Control |
Standard 18605 protocol |
1, 2 |
|
Experimental Group I |
18605 protocol with DNase I treatment added after binding and supernatant removal |
3, 4 |
|
Experimental Group II |
18605 protocol with 1% Tween-20 + 1% Triton X-100 added to Protein Removal Buffer (PE) |
5, 6 |
|
Experimental Group III |
18605 protocol with 2% Tween-20 + 2% Triton X-100 added to Protein Removal Buffer (PE) |
7, 8 |
Results
|
Sample ID |
Sample Type |
Cell Number (approx.) |
RNA Concentration (ng/μL) |
A260/A280 |
A260/A230 |
|
1 |
Vero cells |
~1 × 10⁶ cells |
211.34 |
1.96 |
1.81 |
|
2 |
Vero cells |
~1 × 10⁶ cells |
231.31 |
1.96 |
1.91 |
|
3 |
Vero cells |
~1 × 10⁶ cells |
187.02 |
2.00 |
2.30 |
|
4 |
Vero cells |
~1 × 10⁶ cells |
212.12 |
1.98 |
2.31 |
|
5 |
Vero cells |
~1 × 10⁶ cells |
243.19 |
2.02 |
1.91 |
|
6 |
Vero cells |
~1 × 10⁶ cells |
227.41 |
2.02 |
1.90 |
|
7 |
Vero cells |
~1 × 10⁶ cells |
298.62 |
2.00 |
1.96 |
|
8 |
Vero cells |
~1 × 10⁶ cells |
200.08 |
1.99 |
1.96 |
These results demonstrate that DNase I digestion and surfactant supplementation do not negatively affect RNA yield, purity, or integrity, and can be considered effective strategies to mitigate magnetic bead aggregation when processing high cell-input samples.
Documents
Safety Data Sheet
Manuals
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Расследование
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