The Hieff™ Multiplex PCR Master Mix for NGS is a ready-to-use 2× premix developed for multiplex PCR applications. It contains a hot-start, multiplex-optimized Taq DNA polymerase blend and a proprietary amplification buffer system.

The master mix enables rapid and convenient multiplex PCR and supports efficient amplification of targets with GC content ranging from 25% to 75%. It delivers high uniformity, specificity, and sensitivity across multiple amplicons.

This reagent is suitable for multiplex amplification of 30–300 amplicons of different sizes and can also be used for capture-based assays involving thousands or more amplicons. It is widely applicable in animal and plant breeding, forensic identification, genetic disease testing, food safety analysis, and related fields.

Features

• Easy to operate: Ready-to-use 2× premixed solution
• Broad Compatibility: Suitable for complex samples, GC-rich/poor systems(25-75%), and multiplex amplification.
• Excellent performance: High uniformity, specificity, and sensitivity; supports 30–300 multiplex amplicons or enrichment of thousands of targets.

Components

Storage

This product should be stored at -25~-15℃ for 2 years.

Application

• Targeted gene panels: Amplify disease-related genes (e.g., cancer, inherited disorders).
• Direct blood PCR: No DNA extraction needed.
• Forensic identification: Type STRs/SNPs for identity.
• Agricultural breeding: Detect trait-associated markers (e.g., SNPs, indels).

Figures

1. High Amplification Efficiency  

 

Figure 1. Multiplex amplification test using 13606ES for 300-plex SNP genotyping. Amplicons (100–200 bp) show high specificity and uniformity across all targets.

Documents:

Safety Data Sheet

13606_MSDS_HB260122_EN.PDF

Manuals

13606_Manual_Ver.EN20260127.pdf