The 16S (V4) Bacterial Amplicon Library Preparation Kit enables high-throughput sequencing-based amplification of the V4 region of the 16S rDNA gene. Combined with bioinformatics analysis, this kit allows detection of microorganisms (primarily bacteria) in specific environmental samples, enabling identification of bacterial species and providing comprehensive information on taxonomic classification, species abundance, community structure, phylogenetic relationships, and more.

This kit includes all reagents required for bacterial 16S rDNA amplification. The 16S (V4) primer mix (F/R) amplifies the first ~300 bp fragment of the bacterial 16S rDNA gene. For full-length library construction compatible with MGI™ sequencing platforms, this kit must be used in combination with the Hieff NGS™ Unique Dual Barcode Primer Kit for MGI(Cat# 13350–13353).

All reagents provided in this kit have undergone stringent quality control and functional validation to ensure maximum stability and reproducibility during library preparation.

The 16S rRNA gene is located in the small subunit of the prokaryotic ribosome and consists of 10 conserved regions and 9 hypervariable regions. The conserved regions exhibit minimal variation across bacterial taxa, whereas the hypervariable regions are genus- or species-specific and vary according to phylogenetic relatedness. Therefore, the 16S rDNA sequence serves as a characteristic molecular marker for species identification and is widely regarded as the optimal target for bacterial phylogenetic analysis and taxonomic classification.

16S rDNA amplicon sequencing typically involves selecting one or more hypervariable regions and designing universal primers within flanking conserved regions for PCR amplification, followed by sequencing and analysis of the variable regions for microbial identification. This approach has become a cornerstone method for characterizing microbial community composition in environmental samples. Amplification of the V4 region of 16S rDNA from diverse environmental DNA samples enables discrimination and identification of the vast majority of bacterial taxa and represents the most commonly used standard for bacterial classification.

Features

Broad Sample Compatibility: Supports metagenomic samples ranging from 100 pg to 10 ng, including feces, soil, and extracted meta-DNA.

High Amplification Efficiency: Validated across multiple samples, delivering excellent library yields and high-quality sequencing data.

Consistent Quality: Rigorous batch-to-batch performance and stability controls ensure reliable results.

Components

Storage

Component 12933-A: Store at 2~8 °C;

All other components: Store at –25 ~ –15 °C

Valid life: 12 months

Application

Microbiome Research; Microbial Community Analysis; Environmental Surveillance, Clinical Microbiology; Agriculture-related Microbial Investigations.

Figures

1.Workflow  

Figure 1.Amplicon Library Preparation Workflow

2. High Amplification Efficiency

Figure 2. Amplification performance of the 16S-V4-MGI workflow using 10 ng DNA from soil, fecal, and bacterial samples. First PCR: 25 µL, 28 cycles; second PCR: 100 ng first-round product, 6 cycles. All samples produced successful amplicons with comparable efficiency using Kit 12933.

Documents:

Safety Data Sheet

12933_MSDS_HB251120_EN.PDF

Manuals

12933_Manual_Ver.EN20251120.pdf