40807-RNAi Booster

Next-Gen siRNA Delivery: Achieve >90% Knockdown Efficiency with Lower Reagent Usage —Even in Hard-to-Transfect Cells.

Hieff Trans™ RNAiBoost Transfection Reagent is a next-generation siRNA transfection reagent developed based on an advanced lipids-based platform.
Compared with conventional lipid-based reagents, RNAiBoost achieves >90% gene knockdown in hard-to-transfect cells. RNAiBoost enables efficient transfection in adherent cells, suspension cells, immune cells, and primary cells with a simple and streamlined workflow.

Comparison Indicator	Hieff Trans^TM RNAiBoost	Conventional Transfection Reagents
siRNA Concentration	Low dose (5–50 nM)	High dose (20–100 nM)
Cellular Uptake	Precise delivery with efficient utilization	Large amount enters cells, but most is degraded
Functional siRNA Reaching Cytoplasm	High	Low
Target Gene Knockdown Efficiency	Outstanding (>90%)	Moderate (50–70%)
Post-Transfection Cell Viability	>90%	~70%
Cytotoxicity	Low	Moderate
Medium Change Required	No	Yes

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Key Highlights

High Transfection Efficiency & Broad Cell/RNA Spectrum

Powered by a novel cationic lipid delivery platform, RNAiBoost exhibits enhanced membrane-perturbing and fusion capabilities. It successfully overcomes the endocytosis barriers of traditionally difficult-to-transfect cell types, including primary, immune, and suspension cells. Achieving >90% target gene knockdown, it is universally compatible with siRNA, miRNA mimics/inhibitors, shRNA, and fluorescent RNA oligos.

Advanced Endosomal Escape & Ultra-High Intracellular Utilization

RNAiBoost solves the delivery "last mile" problem. Featuring pH-sensitive membrane disruption, the novel lipids induce membrane rupture or pore formation upon endosomal acidification, enabling the efficient cytosolic release of functional siRNA. This exceptional intracellular utilization ensures that less is more—delivers robust gene silencing at siRNA concentrations as low as 2–50 nM by preventing endosomal entrapment and degradation.

Excellent Cell Viability & Minimal Cytotoxicity

The optimized lipid formulation is engineered to maximize intracellular delivery while prioritizing cell health. Because the system requires a much lower dosage of both reagent and siRNA to achieve optimal knockdown, it significantly minimizes off-target effects and reagent-induced cytotoxicity, maintaining excellent cell viability and physiological relevance.

Fast, Streamlined Workflow & Reliable Reproducibility

Accelerate your research with a simple protocol where complex formation takes as little as 5 minutes. The system is fully compatible with serum- and antibiotic-containing media, requiring no medium replacement. This frictionless workflow dramatically reduces hands-on time while providing the highly consistent, reproducible gene silencing performance required for functional genomics and high-throughput screening (HTS).

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Case Studies and Protocols

Click Cell Name to View Detailed Transfection Protocols

Cell Type	Abbreviation	Nucleic Acid Type
Primary bone marrow-derived macrophages	BMDM	siRNA(6-Well Plate)
Primary bone marrow-derived macrophages	BMDM	siRNA(12-Well Plate)
Primary human umbilical vein endothelial cells	HUVEC	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Human Leukemia T Cell	CEMT-4	siRNA
Human Breast Cancer Cell	MCF-7	siRNA
Human Monocytic Leukemia Cell (induced to M0 Macrophages)	THP-1	siRNA
Human Monocytic Leukemia Cell (induced to M0 Macrophages)	THP-1	siRNA
Human Monocytic Leukemia Cell	THP-1	siRNA
Mouse Lung Carcinoma Cell	LLC	siRNA
Human Histiocytic Lymphoma Cell	U937	siRNA

Cell Type	Abbreviation	Nucleic Acid Type
Human Bronchial Epithelial Cell	BEAS-2B	siRNA
Human Renal Proximal Tubular Epithelial Cell	HK-2	siRNA
Rat Cardiomyocyte	H9c2	siRNA
Human Embryonic Kidney Cell	HEK293	saRNA
Human Hepatic Stellate Cell	LX2	siRNA
Mouse Cardiac Fibroblast	MCF	siRNA
Mouse Embryonic Fibroblast	3T3-L1	siRNA
Mouse Monocytic Macrophage	RAW264.7	siRNA
Mouse Monocytic Macrophage	RAW264.7	siRNA
Rat Bile Duct Epithelial Cell	RBE	siRNA
Mouse Intestinal Endocrine Cell	STC1	siRNA

Cell Transfection FAQs

What type of transfection reagent is this?

This transfection reagent is based on lipids-based technology, enabling efficient intracellular delivery of small RNA molecules with low cytotoxicity.

What cell types is this reagent suitable for?

The reagent is optimized for a broad range of cell types, including: adherent cells, suspension cells, primary cells, stem cells, difficult-to-transfect cells.

Is medium replacement required after transfection?

No. The reagent is fully compatible with serum- and antibiotic-containing media, so medium replacement is generally unnecessary.

What is the recommended siRNA concentration?

For most applications, 5–20 nM siRNA is recommended. Efficient knockdown can often be achieved with concentrations as low as 2 nM.

How long does complex formation take?

Complex formation is rapid and typically completed within 5 minutes at room temperature.

What is the optimal cell confluency for transfection?

For adherent cells, 30–70% confluency at the time of transfection is generally recommended.

How soon can gene knockdown be detected?

mRNA knockdown is typically detectable within 24 hours, while protein knockdown is commonly observed after 48–72 hours.

Does the reagent cause cytotoxicity?

The optimized formulation is designed to minimize cytotoxicity while maintaining high transfection efficiency. Optimization is still recommended for sensitive cell types.

I observed cell death following transfection with RNAiBoost. What should I do?

Remove antibiotics from the culture medium during transfection, as this can induce stress and result in cell death. Try adjusting the quantities of both the lipid reagent and siRNA. Use low-passage cells to ensure better viability. Consider testing for hard-to-detect microbial contaminants, such as mycoplasma.