PEI 40000 is a highly charged cationic polymer with a molecular weight of 40,000 that binds negatively charged nucleic acid molecules very easily, forming a complex and allowing the complex to enter cells. PEI 40000 is a transient transfection reagent with low cytotoxicity, high transfection efficiency, and high gene expression efficiency in cells such as HEK293 and CHO.

PEI 40000 has many advantages over PEI 25000 Transfection Reagent. include:

1. PEI 40000 is easy to dissolve and can be directly dissolved in water. PEI 25000 needs to first adjust the water to weak acid to help it dissolve, and then use NaOH to adjust the pH to neutral.

2. PEI 40000 is easy to operate, easier to use, and has a better transfection effect than PEI 25000; 3. PEI 25000 contains 4-11% propionyl residues, which can prevent the polymer backbone from binding to DNA. Compared to PEI 25000, PEI 40000 is a complete shed construction, so its performance is consistently efficient.

This product is an instant type, which dissolves quickly and is easy to prepare.

Feature

High Transfection Efficiency: Ideal for DNA transfection; transfection efficiency can reach over 80% when cell density is at 70%–80%.

Broad Applications: Suitable for both adherent and suspension cells, and compatible with both transient and stable transfection.

Low Cytotoxicity: Transfected cells maintain good morphology and express high levels of target proteins.

Easy Protocol: Solutions can be prepared directly with distilled water, and complexes can be added directly to serum-containing media.

Cost-Effective: At the same volume/specifications, the price is one-third of the competitor's poly product, making it ideal for large-scale transfection.

Application

Cell Transfection

Specification

Name	Polyethylenimine Linear (PEI) 40000
CAS No.	49533-93-7
Molecular formula	(CH₂CH₂NH)n
Molecular weight	40,000
Solubility	Soluble in water, insoluble in organic solvents: benzene, ether and acetone
Structure
Form	White or off-white solid
Serum Compatible	Yes
Cell Type	Established Cell Lines
Sample Type	Plasmid DNA
Transfection Technique	PEI-Based Transfection

Components

Figures

1. Demonstration of Transfection Effect

Figure 1. The transfection efficiency of Polyethylenimine Linear (PEI) MW40000 Reagent was shown above.

Polyethylenimine Linear (PEI) MW40000 Reagents were used to transfect the HEK293 cell line and CHO-K1 cell line in a 96-well format. GFP expression was analyzed 48 hours posttransfection.

2. Validated Cell Lines

Cited from "TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell . 2022 Mar 17;185(6):980-994.e15. doi: 10.1016/j.cell.2022.02.010. "