Description
Hieff Trans™ mRNA Transfection Reagent is a cationic polymer transfection reagent specifically designed for mRNA cellular transfection, which exhibits high transfection efficiency for routine cells, primary cells, and neural cells. Its unique formulation allows it to be directly added to the culture medium without the serum affecting the transfection efficiency, thus reducing the damage to cells caused by serum removal. There is no need to remove nucleic acid-transfection reagent complexes or change to fresh culture medium after transfection, and fresh culture medium can also be replaced based on the nutritional status of the cells after 4-6 hours.
Features
Stable and Highly Soluble: Colorless and clear; forms RNA-based nanoparticles that remain stable at room temperature for at least 30 minutes, ensuring reproducible experiments and consistent results.
Broad Compatibility: Functions effectively across pH 4–8 and is well-tolerated in physiological buffers and serum, enhancing transfection efficiency in complex environments.
Versatile Applications: Performs excellently with mRNA, siRNA, and gene editing tools, achieving up to 95% gene editing efficiency in vitro.
User-Friendly: Compatible with standard laboratory conditions, requiring no specialized equipment and simplifying experimental procedures.
Applications
mRNA drug development
Gene function research
CRISPR/Cas9 gene editing
Specifications
Form |
liquid |
Nucleic Acid |
mRNA/siRNA/miRNA/ASO |
Category |
In vitro |
Cell |
Primary cells/ Immune cells/ Epithelial cells and other cells. |
Components
Components No. |
Name |
40809ES01 |
40809ES03 |
40809 |
Hieff Trans™ mRNA Transfection Reagent |
0.1 mL |
1 mL |
Shipping and Storage
Hieff TransTM mRNA Transfection Reagent products should be stored at 2-8℃ for 1 year.
Figures

Figure1.A parallel comparison experiment of EGFP mRNA delivery and expression in different cell lines, showing superior performance to competitor T in a variety of cell lines.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.